Specimen: 5 mL (3 mL min.) Whole blood in 2 Lavender Top (EDTA) tubes AND 2 Peripheral blood smears AND 4 mL (2.5 mL min.) Bone marrow Aspirate in 2 Green Top (Sodium heparin) tubes AND 4 Bone marrow aspirate Smears, air dried & fixed in 100% methanol for 30 minutes. Ship at 18-22°C. DO NOT FREEZE. Duly filled Chromosome and FISH analysis Requisition form (Form 17) is mandatory.
Stability (Room): 48 hrs
Stability (Refrigerated): N/A
Stability (Frozen): N/A
Method: Electrical Impedance, VCS, Microscopy, Flow cytometry, FISH, Karyotyping, PCR Fragment Analysis
Price: Contact Medilab
Turnaround time (TAT): 10 – 15 days
Usage: Acute leukemia is broadly classified into Acute myeloid leukemia (AML), Acute lymphoblastic leukemia (ALL) and Acute leukemias of ambiguous lineage (mixed phenotype Acute leukemia and Acute undifferentiated leukemia). This comprehensive panel is on a par with the 2016 Revision to the WHO clssification of Acute leukemia which incorporates morphology, immunophenotype, cytogenetics and molecular study. The morphological evaluation of the blood and bone marrow is followed by flow cytometry with an extended Acute leukemia panel for lineage determination and karyotyping, PCR / FISH for characterization of recurrent genetic abnormalities for a comprehensive diagnosis, personalized / targeted therapy and monitoring.
Components: *CBC *Peripheral blood smear *Bone Marrow Examination Panel 1 *Leukemia diagnostic panel : Acute leukemia – T, B or Myeloid *Cytogenetic / Molecular genetic studies for : AML FISH: t(8;21)(q22;q22.1);RUNX1-RUNX1T1; t(15;17); PML-RARA;inv(16)(p13.1q22)/ t(16;16)(p13.1;q22);CBFB-MYH11; t(9;11)(p21.3;q23.3);MLLT3-KMT2A; PCR – FLT3 & NPM1 gene mutation. ALL FISH: t(12;21)(p13.2;q22.1); ETV6-RUNX1; t(9;22)(q34.1;q11.2);BCR-ABL1; t(1;19)(q23;p13.3);TCF3-PBX1; Karyotyping for hyper/hypo diploidy. MPAL FISH: t(9;22)(q34.1;q11.2);BCR-ABL1
Home Collection: Not Available
Pre-test Information: Duly filled Chromosome and FISH analysis Requisition form (Form 17) is mandatory.